Earticle

초록

영어

Identification of the chemicals that pose a hazard to the developing nervous system is the
first step in reducing human exposure and preventing health problems. In response to the need
for more efficient methods to identify potential developmental neurotoxicants, we designed a cell
based chip to detect the environmental chemical effects on neurite outgrowth in PC-12 cell,
which is a cancer cell line derived from a pheochromocytoma of the rat adrenal medulla. Cell
based chip were fabricated by binding synthetic oligopeptide (RGD) onto a gold electrode. PC-
12 cells were immobilized on the gold electrode. Induction of the neurite cell to grow was
observed by the neurite growth factor (NGF). Electrochemical behavior of the effect of the
environmental chemical (aldrin) on the neurite outgrowth was measured using a cyclic
voltammogram. Voltammetric current peak behavior shows that as the concentration of aldrin
increases, the measured peak current decreases. This indicates a decrease in cell viability. The
cell chip used in this study can be applied to detect the inhibitory effects of a large number of
environmental toxins on neurite outgrowth.

저자

• Rama Bhusal [ Interdisciplinary program of Integrated Biotechnology ]
• Cheol-Heon Yea [ Department of Chemical & Biomolecular Engineering, Sogang Univ. ]
• Waleed Ahmed El-Said [ Interdisciplinary program of Integrated Biotechnology ]
• Il Keun Kwon [ Department of Oral Biology, School of Dentistry, Kyung Hee Univ. ]
• Ji Young Lee [ Department of Chemical & Biomolecular Engineering, Sogang Univ. ]
• Jeong-Woo Choi [ Interdisciplinary program of Integrated Biotechnology, Department of Chemical & Biomolecular Engineering, Sogang Univ. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576