Bio-butanol has become one of the possible alternatives to gasoline fuel. Its better haracteristics such as higher energy content, less hydroscopic and volatile which make it possible to use pre-existing transport infrastructure, than ethanol is regaining the reputation of conventional ABE (Acetone-Butanol-Ethanol) fermentation. However, the potent toxicity of butanol on the main ABE producers derived from Clostridium acetobutylicum ATCC 824, Clostridium beijerinckii NCIMB 8052, Clostridium saccharoperbutylacetonicum N1-4 etc., is still a main drawback of commercialization as a fuel. Extensive studies have been done only to reach the best titer around 19.2 g/L. In this study, we developed new HTS (High-Throughput-Screening) systems specific to butanol based on designed enzymatic reaction. Along with HTS system, we designed a programmed chemical mutagenesis system suitable for screening strains resistant to membrane toxicity. Using our original HTS system coupled with programmed random mutagenesis, we successfully developed a mutant strain, Clostridium beijerinckii CBP-381, which produced at least 30% more butanol under the optimized fermentation condition.
저자
Kwang Myung CHO [ Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology ]
Seung Hwan LEE [ Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology ]
Bong Keun SONG [ Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동