Lowering culture temperature has become popular in a CHO cell culture for improving recombinant protein production and quality. Many reports showed that a reduction in culture temperature (28°C-32°C) resulted in growth arrest, metabolism decrease and improved cell viability with variable effects on recombinant protein productivity. Most published studies revealed lowering culture temperature enhanced specific productivity1, qp, while a few demonstrated no increase in qp at low culture temperature2. Recently, it has been shown that enhancing effect of low culture temperature on qp depends on clones3. Therefore, the selection of clones displaying enhanced qp at low culture temperature is critical for improvement of recombinant protein production. In addition to enhanced qp, lowering culture temperature is known to be advantageous for improving protein quality4 and reducing protein aggregation5. Owing to its various beneficial effects, the application of low culture temperature to a CHO cell culture will be more attractive.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
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