We investigated the effect of a novel phytosphingosine derivative, N-acetyl phytosphingosine (NAPS), on inhibition of α-MSH (melanocyte-stimulating hormone) induced-melanogenesis in B16F1 melanoma. At non-cytotoxic concentrations, NAPS decreased the contents of melanin overproduced by α-MSH induction in both intracellular and the secreted form of it. Furthermore, we observed that NAPS reduced the activity of intracellular tyrosinase increased by α-MSH. Accordingly, we examined whether NAPS could have influence on the expression of melanogenic proteins. Western blot analysis demonstrated that NAPS decreased the expression of tyrosinase and its key transcription factor, microphthalmia-associated transcription factor (MITF), in a concentration-dependent manner. Taken all together, NAPS showed the inhibitory effect on α-MSH-induced melanogenesis through the down-regulation of MITF-tyrosinase expression. Thus, it suggests that NAPS may show skin whitening effect, exerting anti-melanogenic activity as a potent hypopigmenting agent.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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