Earticle

초록

영어

Stationary phase expression system has promising potential for production of toxic proteins such as antimicrobial peptide in Escherichia coli. Here we characterized and evoluted the promoter of ptsL, a gene identified by 2-D gel to be expressed from the stationary phase. The promoter region of ptsL is fused with the gene encoding enhanced green fluorescence protein (EGFP) to check the characteristic of stationary phase expression of this system by analyzing the fluorescence generated by cells. Furthermore, mlc which is encoding a repressor of ptsL and led by pBAD promoter to control its expression by different dose of arabinose, is cloned into the same plasmid. The purpose of introducing mlc gene here is to inhibit possible leak expression of EGFP prior to stationary phase, which is privotal when this system is used to produce toxic proteins. Finally, directed evolution was applied to enhance the expression level of ptsL promoter.
[This work was supported by the Korean Systems Biology Research Program (M10309020000-03B5002-00000) of the Ministry of Science and Technology]

키워드

저자

• Shun Zheng [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST ]
• Tae Jung Park [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, BioProcess Engineering Research Center, Systems and Synthetic Biology Research Center, Institute for BioCentury, and Center for Ultramicrochemical Process Systems, KAIST ]
• Xiao Xia Xia [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST ]
• Hyun Uk Kim [ Department of Chemical & Biomolecular Engineering (BK21 program), KAIST ]
• Sang Yup Lee [ BioProcess Engineering Research Center, Systems and Synthetic Biology Research Center, Institute for BioCentury, and Center for Ultramicrochemical Process Systems, KAIST, Department of Bio & Brain Engineering, and Bioinformatics Research Center, KAIST ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576