the purpose of this study was to assess the effects of a herb remedy . on the cell proliferation, inducible nitric oxide synthase (inos) mRNA gene expression and nitric oxide (NO) production in RAW 264.7 macrophage cells. For the screening of anti-inflammation, a ethanolic extract of traditional herbal medicine was examined its inhibitory effect, and we confirmed that 1 herb. NO concentrations were determined by measuring the amount of nitrite in the LPS treated-macrophage cell culture supernatant using Griess reagent. In cytotoxicity test, with the a herbal remedy concentration of up to 500 mg/mL, no significant effect on RAW 264.7 macrophage cells morphology and viability was observed compared to the control during the culture period. Also, more than the concentration of 125ppm, a herbal remedy was significantly decreased the production of NO. As a result, we knew inhibitory effects of a herbal remedy extracts on Nitric Oxide synthesis in RAW 264.7 macrophage cells.
저자
Hye-Sung Youm [ department of biological engineering, Incheon ]
Tae- Ryeon Heo [ department of biological engineering, Incheon ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동