Byung-Kyun Kim, Ja-Ryong Koo, Kyuboem Han, Eung-Soo Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98966
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원문정보
초록
영어
The polyene antifungal antibiotics, mostly produced by Gram-positive soil actinomycetes, are a family of type I polyketide macrolide ring compounds with 20-40 carbon backbone contain 3-8 conjugated double bonds. Using polyene-specific genomic screening strategy, we previously isolated three novel polyene-producing actinomycetes strains from soil, implying the potential application of these strains’ spores as microbial pesticides. Among three isolated strains, an approximately 112.9 kb contiguous nystatin-like CPP (Cryptic Pseudonocardia Polyene) biosynthetic gene cluster in P. autotrohpica and a 44.8kb FR008-like polyene biosynthetic gene cluster in Streptomyces MMBL003 have been isolated and genetically characterized. Using a fractional factorial design, both KH2PO4 and yeast extract were identified to be the key factors influencing CPP productivity in P. autotrophica. In addition, we confirmed that the sonication was a very efficient method for actinomycetes spore generation with a sonicator power-dependent manner. These sonication-driven actinomycetes spores retained significant portion of their cell viabilities as well as antifungal activities after freeze-drying procedure, implying the potential application of these strains’ spores as microbial pesticides.
저자
Byung-Kyun Kim [ Department of Biotechnology, Inha University ]
Ja-Ryong Koo [ Department of Biotechnology, Inha University ]
Kyuboem Han [ Hanson Biotech Co., Ltd. ]
Eung-Soo Kim [ Department of Biotechnology, Inha University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동