Earticle

초록

영어

The early diagnosis of bovine pregnancy is an essential component of successful reproductive planning on farms, because lack of bovine pregnancy over the long term results in reproductive failure and low milk yield‐the latter of which is a special concern on dairy farms. This study was designed to identify early pregnancy‐specific whey proteins in bovine, by comparing milk samples collected from cattle during pregnancy (Days 30 and 50) and from non‐pregnant cattle. In this study, differentially expressed proteins in five pregnant and five non‐pregnant Holstein dairy cattle were investigated and compared, using proteomics analysis. The first dimension was applied to a pH 3.0～10.0 strip, by loading a 2‐mg milk protein sample. After the second‐dimension separation was performed, the gels were stained with colloidal Coomassie brilliant blue. The stained gels were scanned and the images were analyzed, to detect variations in protein spots between non‐pregnant and pregnant cattle milk protein spots, using ImageMaster; this was followed by analysis with MALDI TOF‐MS. Analysis of the 2‐DE gel image resulted in a total of approximately 500～600 protein spots, of which 12 spots were differentially expressed, six spots were up‐regulated, and four spots were downregulated; two spots were identified as pregnancy‐specific proteins. These proteins were identified as lactoferrin, NADH dehydrogenase subunit 2, albumin, serum albumin precursor and transferrin. Our results via 2‐D PAGE analysis revealed composite profiles of several milk proteins related to early bovine pregnancy, implying the possible use of these milk proteins in the early detection of bovine pregnancy.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Rong Xun Han [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Su Min Choi [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Myung Youn Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Yan Shi Quan [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Baek-Chul Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Yun Fei Diao [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Reza Koqani [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Chang Sik Park [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ]
• Dong Il Jin [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University, Daejeon 338-708, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636