Jin seop Ahn, Jung Ki Yoo, Hyun Min Jung, Jin Kyeoung Kim, Sung-Hwan Moon, Hyung-Min Chung
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98928
※ 기관로그인 시 무료 이용이 가능합니다.
※ 학술발표대회집, 워크숍 자료집 중 4페이지 이내 논문은 '요약'만 제공되는 경우가 있으니, 구매 전에 간행물명, 페이지 수 확인 부탁 드립니다.
4,000원
원문정보
초록
영어
Human embryonic stem (ES) cells retain the capacity for self‐renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf‐ 31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord‐blood endothelial progenitor cells (CB‐EPCs) and somatic cell lines, we performed RT‐PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40‐immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES
Jin seop Ahn [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
Sung-Hwan Moon [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-907, Korea ]
Jung Ki Yoo [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
Hyun Min Jung [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
Hyung-Min Chung [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-907, Korea ]
Jin Kyeoung Kim [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
Corresponding author