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Expression of the C1orf31 Gene in Human Embryonic Stem Cells and Cancer Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 4 (2008.12)바로가기
  • 페이지
    pp.223-227
  • 저자
    Jin seop Ahn, Jung Ki Yoo, Hyun Min Jung, Jin Kyeoung Kim, Sung-Hwan Moon, Hyung-Min Chung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A98928

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초록

영어
Human embryonic stem (ES) cells retain the capacity for self‐renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf‐ 31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord‐blood endothelial progenitor cells (CB‐EPCs) and somatic cell lines, we performed RT‐PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40‐immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

저자

  • Jin seop Ahn [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
  • Sung-Hwan Moon [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-907, Korea ]
  • Jung Ki Yoo [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
  • Hyun Min Jung [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ]
  • Hyung-Min Chung [ Stem Cell Research Laboratory, CHA Stem Cell Institute, Pochon CHA University, Seoul 135-907, Korea ]
  • Jin Kyeoung Kim [ Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Kyungki-do 463-836, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 32 No 4

    피인용수 : 0(자료제공 : 네이버학술정보)

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