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Effect of Various Caffeine Concentrations and Fertilization Time in In Vitro Fertilization of Canine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 4 (2008.12)바로가기
  • 페이지
    pp.217-222
  • 저자
    Bong Soo Kim, Dong Hoon Yoo, Mi Jung Shin, Hae Jung Kim, Dong Seok Lee, Sanggyu Lee, Ji Hong Ha, Zae Young Ryoo, Byung-Hwa Hyun, Young Sik Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A98927

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원문정보

초록

영어
The techniques of IVM, IVF and IVC of canine oocytes may provide useful information for gamete salvage programs and the conservation of endangered canidae. This investigation has been made to determine the efficiency of in vitro maturation (IVM) as a basic experiment to study the development of canine oocytes after in vitro fertilization (IVF). The rate of oocytes developing to the MII stage was higher in the hormone treated group (10 IU/ml hCG+eCG, 14.7%, p<0.05) than in the control group (0 IU/ml hCG+eCG, 10.0%). The monospermy and pronuclear rates of canine oocytes were investigated after caffeine treatment on IVF. Canine oocytes were fertilized in the Fert‐TALP medium supplemented with 0, 10, 20 or 30 mM caffeine (Fert I, Fert II, Fert III or Fert IV, respectively). The highest pronuclear formation rate was obtained in the Fert I for 24 h IVF (6.7%, 6/89). Therefore, it is believed that unlike in other mammals, caffeine in canine IVF does not increase the efficiency of fertilization rate, and is not an important factor.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Canine Oocyte In vitro maturation In vitro fertilization Sperm capacitation

저자

  • Bong Soo Kim [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Dong Hoon Yoo [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Mi Jung Shin [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Hae Jung Kim [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Dong Seok Lee [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Byung-Hwa Hyun [ Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea ]
  • Sanggyu Lee [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Young Sik Park [ Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701, South Korea ]
  • Ji Hong Ha [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ]
  • Zae Young Ryoo [ School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-Dong, Buk-Ku, Daegu 702-701 South Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 32 No 4

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