Earticle

초록

영어

To produce an important platform chemical 3-hydroxypropionic acid (3-HP) from glycerol, we
developed a recombinant E. coli BL21 strain by cloning two sets of genes; dhaB and gdrAB under
T7 promoters with pCDFDuet vector; and the gene KGSADH under pRSFDuet vector: In which
the gene dhaB encodes an enzyme glycerol dehydratase of Klebsiella pneumoniae, capable of
converting glycerol to 3-hydroxypropionaldehyde (3-HPA); and the genes gdrAB of K.
pneumoniae which encode the reactivation factor of glycerol dehydratase, whilst the gene
KGSADH of Azospirillum brasilense encodes γ-ketoglutaric semialdehyde dehydrogenase, that is
capable of converting 3-HPA to 3-HP. When analyzing the crude-cell extracts of recombinant
proteins, DhaB exhibited 95 U/mg protein on glycerol with coenzyme B12, while the enzyme
KGSADH exhibited 2.9 U/mg protein on 3-HPA in the presence of NAD+ as hydrogen acceptor.
Upon cultivating the recombinant on a glycerol medium under aerobic condition, it produced a
maximum of 19.6 mmol 3-HP or 1.76 g per liter at 0.5 mM IPTG induction. The rate and yield of
3-HP production were estimated as 1.1 mmol g-1 cdw h-1 and 0.28 mol mol-1 glycerol,
respectively. Although the final titer of 3-HP is too low for commercial applications, our results
in the present investigation demonstrate the utility of the recombinant for further development of
a bioprocess to improve 3-HP from glycerol.

저자

• Chelladurai Rathnasingh [ Department of Chemical and Biochemical Engineering, Pusan National University ]
• S. Mohan Raj [ Department of Chemical and Biochemical Engineering, Pusan National University ]
• Ji-Eun Jo [ Department of Chemical and Biochemical Engineering, Pusan National University ]
• SungHoon Park [ Department of Chemical and Biochemical Engineering, Pusan National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576