Earticle

초록

영어

Polyunsaturated fatty acids (PuFAs) like EPA and DHA have attracted public attention because of their beneficial effects in human health. To date, several genes encoding critical enzymes like desaturases and elongases involved in the biosynthesis of PuFAs have been cloned from various organisms. A TaELO elongase gene was isolated from Thraustochytrium aureum ATCC 34304 and cloned into Pichia pastoris GS115 using pPIC3.5 vector. C18-20 elongase and C20-22
elongase was expressed through multistep in P. pastoris GS115. The temperature is an important parameter for optimization of elongase expression in yeast systems. In this report, we demonstrated the effects of cultivation temperature on TaELO elongase gene expression in C18-20 elongation step.

저자

• Myung-Ju Noh [ Department of Marine Science & Biological Engineering, College of Engineering, Inha University, Institute of Biotechnological Industry, College of Engineering, Inha University ]
• Jae-Cheol Lee [ Department of Marine Science & Biological Engineering, College of Engineering, Inha University, Institute of Biotechnological Industry, College of Engineering, Inha University ]
• Won-Ho Kim [ Institute of Biotechnological Industry, College of Engineering, Inha University ]
• Byung-Ki Hur [ Department of Marine Science & Biological Engineering, College of Engineering, Inha University, Institute of Biotechnological Industry, College of Engineering, Inha University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576