Earticle

초록

영어

Mannheimia succiniciproducens MBEL55E, a capnophilic rumen bacterium, produces succinic acid in high concentrations under anaerobic conditions. In this study we investigated the response of M. succiniciproducens MBEL55E to different levels of dissolved CO2 ranging from 0 to 260 mM. Comparing the fermentation results with the levels of key enzyme activities, we found that the efficient carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate, in which
CO2-fixation occurs by both PEP carboxykinase (PckA) and PEP carboxylase (Ppc), allows M. succiniciproducens to produce substantial amounts of succinic acid. In particular, the activity of PckA was more than 50 times higher than those of Ppc and malic enzyme (SfcA). The results provide useful information for the design of effective bioprocesses to improve succinic acid production.
[This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].

저자

• Sol Choi [ Department of Chemical and Biomolecular Engineering (BK21 program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST ]
• Hyohak Song [ Department of Chemical and Biomolecular Engineering (BK21 program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, BioProcess Engineering Research Center, KAIST, ]
• Jeong Wook Lee [ Department of Chemical and Biomolecular Engineering (BK21 program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST ]
• Joungmin Lee [ Department of Chemical and Biomolecular Engineering (BK21 program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST ]
• ang Yup Lee [ Department of Chemical and Biomolecular Engineering (BK21 program), Metabolic and Biomolecular Engineering National Research Laboratory, KAIST, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, BioProcess Engineering Research Center, KAIST, Department of Bio and Brain Engineering, BioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576