Earticle

초록

영어

Chemical synthesis of 1,2-propanediol (1,2-PD, propylene glycol) that is most commodity chemicals have some problems [1]. In this work, microbial fermentation was used form the production of useful industrial chemicals [2]. Saccharomyces cerevisiae was selected a host strain. Methylglyoxal synthase (mgs) and glycerol dehydrogenase (gldA), the two enzymes were selected for the production of 1,2-PD metabolism. And the two enzymes that are essential
intermediate from DHAP and glycerol dehydrogenase [2-4]. The yeast expression vector, pESC-URA has multi-cloning sites, was used construction plasmid containing target gene mgs and gldA. The plasmid containing mgs and gldA was inserted into S. cerevisiae by LiAc/SS-carrier DNA/PEG transformation. And then strain containing plasmid was screened by SDC (A,T)
medium. The culture medium was YPD adding galactose as trigger for the activation of GAL promoter in the plasmid [5]. The concentration of 1,2-PD was analyzed by HPLC with refractive index detector. The mobile phase was 0.5M sulfuric acid and flow rate was 0.5 ml/min. After growing the yeast, we broke the cells to extract 1, 2-PD. The solution for 1,2-PD analysis was condensed and analyzed by HPLC. Cells containing mgs or gldA or two genes in pESC-URA vector were shown the production of 1,2-PD.

저자

• Yong Ho Noh [ Dept. of Biological Engineering, Inha University ]
• Se Kyung Kim [ Dept. of Biological Engineering, Inha University ]
• Bong Je Park [ Dept. of Biological Engineering, Inha University ]
• Jin Won Lee [ Dept. of Chemical Biomolecular Engineering, Sogang University ]
• Hyun Shik Yun [ Dept. of Biological Engineering, Inha University ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576