D- and L-lactic acid are optical isomers, which are formed by distinct stereo-specific NAD-dependent lactate dehydrogenases (LDH) in lactic acid bacteria(LAB). Leuconostoc (Ln.), the major bacterial population in kimchi, has been predicted to synthesize only D-lactic acid from pyruvate, as they harbor a single D-LDH gene but L-LDH gene. D-lactic acid may lead to acidosis in infants and in adults with short bowel syndrome. As one of the major D-lactic acid producers in kimchi, it is required to switch the major lactate isoform of Ln. citreum from D- to L-. Hence, with the above long-term goal, first, we have determined the profiles of D-/L- lactic acid synthesis of LAB culture broth. Secondary, we cloned ldhL gene from Lactobacillus plantarum and expressed it in Ln. citreum. Finally, the characteristics of the recombinant Ln. citreum were investigated.
저자
Qing Jin [ Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University ]
Jee Yun Jung [ Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University ]
Hyun-Ju Eom [ Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University ]
Nam Soo Han [ Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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