JI Song Jo, Bo Mi Ku, Jae Ran Lee, You Geun Kim, Sang Soo Kang, Sung Bae Kim, Seon-Won Kim, Chang-Joon Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98688
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원문정보
초록
영어
Natural -carotene has been used as a functional β cosmetic agent because chemical synthetic one has been severely regulated in cosmetic industry. Although anti-wrinkle properties of β-carotene has reported, no experimental evidences and/or optimal dosage were not revealed. In this presentation, we described the extraction of beta-carotene from metabolically engineered E.coli, purification by crystallization, and evaluation of anti-wrinkle efficacy of purified beta-carotene. 223 g/L of wet cells was harvested from 2.5-L fed-batch culture broth. After cell disruption by sonication, dehydration using acetone, extraction with isobutyl acetate at 60oC for 10 min. and crystallization of cell extracts at 4oC for 12 h. 633 mg of β-carotene was obtained with a purity of 93% as measured by HPLC. Isolated beta-carotene was evaluated by performing collagen synthesis assays using human normal fibroblast cells. 3.5 μM of beta-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of beta-carotene. This indicates that beta-carotene derived from recombinant E.coli can be used in functional cosmetics as anti-wrinkle agent.
저자
JI Song Jo [ Department of Chemical & Biological Engineering, GyeongSang National University ]
Bo Mi Ku [ Department of Anatomy & Neurobiology, GyeongSang National University, Jinju, Korea ]
Jae Ran Lee [ Department of Chemical & Biological Engineering, GyeongSang National University ]
You Geun Kim [ KB Cosmetics, Jinju, Korea ]
Sang Soo Kang [ Department of Anatomy & Neurobiology, GyeongSang National University, Jinju, Korea ]
Sung Bae Kim [ Department of Chemical & Biological Engineering, GyeongSang National University ]
Seon-Won Kim [ Division of Applied Life Science, GyeongSang National University, Jinju, Korea ]
Chang-Joon Kim [ Department of Chemical & Biological Engineering, GyeongSang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동