Earticle

초록

영어

One of the serious problems concerning water in the environment is its pollution with naturally occurring toxins presumably produced by cyantobacteria (blue-green algae) that produce algae blooms. The cyanobacteria produce various types of toxins. One of them is microcystin, a hepatotoxin. Microcystins from cyanobacteria are released when the cells are lysed, die and break open. The incidents of wild and domestic animal poisoning and human health problems attributed to exposure to microcystins have been well documented. So for health risks assessment from the microcystins, the rapid system to detect and monitor microcystins is required. Quantum-dot nanocrystals have high fluorescence efficiency, lack of photobleaching, and long fluorescence lifetimes. Here we biocojugated quantum-dot 525 (Invitrogen) to the monoclonal microcystin antibody to detect the microcystins extracted from cultured Microcystin aeruginosa (NIER 10038). When quantum-dot 525 antibody conjugates interact with the microcystins, the fluorescence emission spectra and intensities changes were observed using a fluorescence spectrophotometer.

키워드

저자

• Jinwook Lee [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
• Hye-weon Yu [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]
• In S. Kim [ Bio-Environmental Engineering Lab., Dept. of Environmental Science and Engineering, Gwangju Institute of Science and Technology ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576