Earticle

초록

영어

This study describes formation of Poly(ethylene glycol) (PEG) hydrogel nanofibers via electrospinning, which can be used as a scaffold for 3-dimensional cell culture system.
Currently, most of in vitro cell-based assays for drug screening and toxicity evaluation were
performed using cells adhered on the 2-dimensional substrates. However, in a 2-dimensional system, cells are in an unnatural environment; I.e., in tissue they exist in a 3-dimensional hydrogel nanofiber matrix consisting of proteins and polysaccharides (i.e., the extracellular matrix). As a result, the response of these cells to drug candidates may be very different than that of the same cells in their native tissue. To overcome thes problems and mimic the native tissue, PEG hydrogel nanofiber was prepared by electrospinning PEG precursor solution containing poly(ethylene oxide) (PEO) and thermal or photo-initiators. Without PEO, electrospinning of PEG solution produced microsphere instead of fiber due to low viscosity. Addition of high molecular weight of PEO made it possible to create nanofiber and resultant nanofiber was crosslinked thermally or photochemically to prevent dissolution of nanofiber in aqueous environment. Formation of PEG hydrogel nanofiber was confirmed using SEM and other characterization including water content and mechanical strength were carried out varying the molecular weight of PEG.

저자

• Dae Nyun Kim [ Department of Chemical Engineering, Yonsei University ]
• Hyun Jong Lee [ Department of Chemical Engineering, Yonsei University ]
• Woojin Lee [ Department of Chemical Engineering, Yonsei University ]
• Yeol Lee [ Department of Chemical Engineering, Yonsei University ]
• Eun Ji Jang [ Department of Chemical Engineering, Yonsei University ]
• Sang Phil Park [ Department of Chemical Engineering, Yonsei University ]
• Saemi Park [ Department of Chemical Engineering, Yonsei University ]
• Wongun Koh [ Department of Chemical Engineering, Yonsei University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576