Hyun Woo Jo, So Ra Kim, Sang Youn Hwang, Eun Kyu Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98633
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
LAL (Limulus amebocyte lysate) test is used to detect bacterial endotoxin or LPS (lipopolysaccharide), a membrane component, of gram negative bacteria. There are 3 methods available for conventional testing: gel clotting, KTA (kinetic turbidimetric assay) and KCA (kinetic chromogenic assay). These require long incubation and expensive reagents. And LOC (lab-on-a-chip) is one of the solutions. We evaluated the assays' feasibility in terms of reducing assay time and volume. Among the 3, gel clotting was excluded because its result was too rough to be quantitative. The volume of LAL and CSE (control standard endotoxin) mixture was from 50 to 200 μl, and its final concentration was from 0.05 to 5 EU/ml. The test was triplicated. 96-well plate reader read the absorbance of a well every 3 min during 60±1 min at 340 nm for KTA, and 405 nm for KCA with 37±1°C. Absorbance per light path length of each reaction volume was calculated. Absorbance controlled regression analysis showed the coefficient of determination above 0.98. These data suggested the assay time was shortened by 1/2 hr than the conventional methods and the LOC could distinguish various concentration of samples.
저자
Hyun Woo Jo [ Department of Material Science & Engineering ]
So Ra Kim [ Department of Chemical Engineering, Hanyang University ]
Sang Youn Hwang [ Department of Chemical Engineering, Hanyang University ]
Eun Kyu Lee [ Department of Chemical Engineering, Hanyang University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동