Spore-forming bacterium Bacillus anthracis is cause of anthrax which is an acute infectious disease. Anthrax most commonly occurs in mammal such cattle, sheep, goats, camels and antelopes, but can also occur in humans when they are exposed to infected animals or tissue from infected animals. The goal of this research is to propose new experimental ideas that may serve detection method of B. subtilis which is similar with B. anthracis by surface plasmon resonance (SPR). Surface construction provided by R. A. Vega et al. previously was used. After immobilization of poly- and monoclonal antibodies with metal ions on gold thin film, response of a layer of antibody in the presence of microbes is recorded through the shift of SPR angle. In this report, passivation layer of thiolated poly-ethylene glycol (PEG-SH) is suggested to improve sensitivity and to get optimum antibody orientation. Relatively uniform immobilization of antibody structures in active states leads to abundant possibility of efficient measurement of B. subtilis.
저자
Sira Lee [ Department of Chemical and Biomolecular Engineering, Sogang university ]
Byung-Keun Oh [ Department of Chemical and Biomolecular Engineering, Sogang university, Interdisciplinary Program of Integrated Biotechnology, Sogang university ]
Jinwon Lee [ Department of Chemical and Biomolecular Engineering, Sogang university, Interdisciplinary Program of Integrated Biotechnology, Sogang university ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동