Earticle

초록

영어

In this work, we first report self-assembly of insulin monomers into amyloid fibrils on a solid surface within microchannel by using microfluidics. To demonstrate amyloid formation and growth on a solid surface, we prepared NHS-activated glass and PDMS film embossed with a microchannel. According to the analysis with optical and fluorescence microscope, amyloid aggregates within microchannels were found to be increased with the duration of incubation
due to the continuous flow of insulin monomers. We confirmed numerous amyloid fibrils grown inside microchannels through AFM analysis and the density of fibrils was much higher than those formed in other bulk systems. We observed the formation of spherulites showing “maltese cross” patterns inside the microchannel after 12 h. These results indicate that the microfluidic
self-assembly into amyloid fibrils enables the low consumption of reagents, reduction in incubation time for amyloid formation, and a simultaneous detection.
The microfluidic system for amyloid formation on the surface has a potential contribution in the field of study of the amyloidogenic peptides that are responsible for different neurodegenerative disease.

저자

• Joonseok Lee [ Department of Materials Science and Engineering ]
• Chan Beum Park [ Department of Materials Science and Engineering ]
• Eujin Um [ Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology ]
• Je-Kyun Park [ Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576