The ability to organize multiple biomolecules on micro/nanometer scale pattern is crucial for the development of biological integrated devices such as DNA/protein chip. In this study, a gold pattern on silver substrate was prepared. A novel and ultrasensitive detection method of prostate specific antigen (PSA) by scanning tunneling microscopy (STM) was developed on the micro gold pattern. PSA is a marker for prostate cancer and it has been identified as a potential marker for breast cancer in women. The detection method is based on the change of tunneling current given by the gold nanoparticle dispersed on micro gold pattern insulated with biomaterial. Biomolecular thin film on the pattern and the Au nanoparticle-antibody conjugate were fabricated in an oriented manner with the antibody fragments. As a result, the frequency of current peaks was generated in accordance with the surface density of the dispersed Au nanoparticle on pattern. And, the change of the power spectrum was observed in accordance with the concentration of PSA molecule. The lowest detection limit of the assay system for PSA was 33fM (10pg/mL).
저자
Da-Yeon Kang [ Department of Chemical & Biomolecular Engineering, Sogang University ]
Yong-Hark Jang [ Department of Chemical & Biomolecular Engineering, Sogang University ]
Jin-Ho Lee [ Department of Chemical & Biomolecular Engineering, Sogang University ]
Byung-Keun Oh [ Department of Chemical & Biomolecular Engineering, Sogang University, Interdisciplinary Program of Integrated Biotechnology, Sogang University ]
Jeong-Woo Choi [ Department of Chemical & Biomolecular Engineering, Sogang University, Interdisciplinary Program of Integrated Biotechnology, Sogang University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동