Eun Hae Oh, Sang Hun Lee, Jong Hwan Sung, Hyun Seok Song, Tai Hyun Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98619
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Recently, many commercial electronic noses have been developed and are being applied to such areas as food, beverage, agriculture, health, industry and environment. But the electronic noses have limitation; less sensitivity, high cost, large equipment and so on. Biosensor using olfactory receptor can overcome these limitation having high sensitivity and specificity. In previous study, quartz crystal microbalance (QCM) was used to check olfactory receptor protein ODR10 interact with diacetyl. Such system could be applied to test the interaction of human olfactory receptor and specific odorants. For this experiment, Human olfactory receptor OR52D1 interacting with ethyl isobutyrate is chosen. OR52D1 was expressed in E.coli as a fusion protein with GST tag and the expression was confirmed through western blot using anti-GST antibody. Crude membrane extract of the expressed protein was coated on the surface of gold, and the interaction of the olfactory receptor and specific odorant was examined. OR52D1 interacted with isobutyrate stronger than other odorants is confirmed by QCM.
저자
Eun Hae Oh [ School of Biological and Chemical Engineering, Seoul National University ]
Sang Hun Lee [ School of Biological and Chemical Engineering, Seoul National University ]
Jong Hwan Sung [ School of Biological and Chemical Engineering, Seoul National University ]
Hyun Seok Song [ School of Biological and Chemical Engineering, Seoul National University ]
Tai Hyun Park [ School of Biological and Chemical Engineering, Seoul National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동