Yoon-Suk Kang, Yunho Lee, Jin Hwan Song, Woojun Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98608
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원문정보
초록
영어
To alleviate oxidative stress generated during naphthalene metabolism, overexpression of antioxidant enzymes [Fpr (ferredoxin-NADP+ reductase) and SOD (superoxide dismutase)] was conducted in naphthalene-degrading bacterium Pseudomonas sp. strain As1. The fpr and sodA gene was placed under the transcriptional control of either constitutive lac promoter or native promoter. Both high performance liquid chromatography (HPLC) analysis and growth rate have shown that the recombinant cells effectively degraded naphthalene compared to wild type. All recombinant cells, except a strain As1(sodA), where sodA gene was up regulated by the lac promoter, exhibit more resistance to a superoxide generating agent, paraquat. Growth of As1(sodA) was inhibited by paraquat and its growth defect were significantly overcome by alkyl hydroperoxide reductase overproduction. Taken together, the data demonstrated that overproduction of these two genes contribute to oxidative tolerance during naphthalene degradation, however, more than necessary SOD, which may provoke generation of hydrogen peroxide, results in cell death.
저자
Yoon-Suk Kang [ Division of Environmental Science and Ecological Engineering, Korea University ]
Yunho Lee [ Division of Environmental Science and Ecological Engineering, Korea University ]
Jin Hwan Song [ Division of Environmental Science and Ecological Engineering, Korea University ]
Woojun Park [ Division of Environmental Science and Ecological Engineering, Korea University, Environmental Biotechnology National Core Research Center, Gyeongsang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동