Earticle

초록

영어

A living cell can be properly described as an electrochemical dynamic system [1]. Many electrochemical methods have been used in studying intact living cells. We use the cyclic voltammetric (CV) and Potentiometric Stripping Analysis (PSA) methods to study the viability of Hela cells and the effect of anticancer drugs. HeLa cells were grown on gold patterned on silicon substrate; they wereused as a working electrode. Counter test was performed to confirm the results obtained from CV. The voltammetric behaviors of HeLa cells showed a quasi-reversible process and the peak current showed a linear relationship with cell number. PSA signal was used to detect the cell growth with time; as the culture time increases the area under the peak increase gradually reaches to amaximum value and then decreases sharply. The initial increase of PSA signal with the culture time is due to the proliferation of cells. In cell culture, nutrients
are important in maintaining cell viability when the cells are cultured continuously in the CO2 incubator for longer time without any fresh nutrient replenished, the viability of cells can undoubtedly be affected. So the decrease of PSA signal in is related to the decrease of cell viability. Using Hydroxyurea and Cyclophosphamide as anticancer drugs, we observed reduction in peak current by increasing in their concentration. The electrochemical experiments on the
cells also were verified in comparison with counter assay test. These results indicate that the voltammetric and PSA methods can be used tostudy the viability of Hela cells and the effect of anticancer drugs. Acknowledgments : This research was supported by Seoul R&BD Program(10816) and by the Nano/Bio science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Science and Technology (MOST) and by the Korea government(MOST) (2006-05374).

저자

• Waleed Ahmed El-Said [ Interdisciplinary Program of Integrated Biotechnology, Sogang University ]
• Sang-Uk Kim [ Interdisciplinary Program of Integrated Biotechnology, Sogang University ]
• Cheol-Heon Yea [ Department of Chemical & Biomolecular Engineering, Sogang University ]
• Jin-Ho Lee [ Department of Chemical & Biomolecular Engineering, Sogang University ]
• Hyunhee Kim [ Department of Chemical & Biomolecular Engineering, Sogang University ]
• eong-Woo Choi [ Interdisciplinary Program of Integrated Biotechnology, Sogang University, Department of Chemical & Biomolecular Engineering, Sogang University ]

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간행물

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576