Tae Jung Park, Sungho Ko, Moon Seop Hyun, Hyun Uk Kim, Sang Yup Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98587
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원문정보
초록
영어
It is very critical to develop the surface plasmon resonance (SPR) sensor in order to achieve a severe acute respiratory syndrome (SARS) coronavirus sensor. This work is specifically focused on the development of biosensor system to obtain recombinant proteins with the gold-specific affinity. The strategy is based on the principle that the gold binding polypeptide (GBP) as a fusion partner specifically binds onto the gold surface for immobilizing proteins. The SARS coronavirus envelope was employed as a model protein for the specific immobilization of GBP-fusion protein. The fusion proteins were successfully immobilized on the gold surface and examined by SPR analysis. We also analyzed the immobilization efficiency of intracellular protein on the micro-patterned gold surface. Finally, the GBP-fusion method immobilized proteins onto the gold surface without surface modification and in a bioactive form, which is suitable for studying interactions of protein-protein, and other biomolecules [This work was supported in part by MIC & IITA through IT Leading R&D Support Project.].
저자
Tae Jung Park [ Department of Chemical & Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Systems and Synthetic Biology Research Center, Institute for BioCentury, and Center for Ultramicrochemical Process Systems, KAIST ]
Sungho Ko [ Food Nanotechnology Research Group, Korea Food Research Institute ]
Moon Seop Hyun [ Measurement & Analaysis Team, National Nanofab Center ]
Hyun Uk Kim [ Department of Chemical & Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Systems and Synthetic Biology Research Center, Institute for BioCentury, and Center for Ultramicrochemical Process Systems, KAIST ]
Sang Yup Lee [ Department of Chemical & Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Systems and Synthetic Biology Research Center, Institute for BioCentury, and Center for Ultramicrochemical Process Systems, KAIST, 4Department of Bio & Brain Engineering, and Bioinformatics Research Center, KAIST ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동