Earticle

초록

영어

Analysis of methanogenic communities in an anaerobic batch system is essential for the process to be better understood and managed. The diversity of methanogenic populations, along with the process data of an anaerobic batch process treating waste activated sludge, was monitored for 25 days. For the understanding of microbial diversity and dynamics, 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting was conducted in
two different taxonomic levels of domain and order. Domain Archaea and three orders Methanosarcinales, Methanomicrobiales, and Methanobacteriales DGGE profiles were comparatively analyzed by sequences identification of each DGGE band. The profiles of three orders DGGE showed new methanogens belonging to each order but they were not detected by the profiles of Archaea DGGE. The divergences could be resulted from PCR bias or different microbial abundancesof each order in anaerobic bioreactor. In conclusion, for the understanding of fully detailed methanogenic diversity and dynamics occurring in anaerobic bioreactor, it is necessary to conduct DGGE analysis by lower taxonomic 16S rRNA gene primer.

저자

• Kwanghyun Hwang [ School of Environmental Science and Engineering, Pohang University of Science and Technology ]
• Seung Gu Shin [ School of Environmental Science and Engineering, Pohang University of Science and Technology ]
• Jaai Kim [ School of Environmental Science and Engineering, Pohang University of Science and Technology ]
• Seokhwan Hwang [ School of Environmental Science and Engineering, Pohang University of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576