Recently terrorist action poses the threat of bio-warfare using potential harmful bacterial spores. The anthrax attack of Oct 2001 in USA demonstrates the need for a rapid detection system for spores of Bacillus anthracis. In this work we have developed bacterial endospore detection technique based on the reaction dipicolinic acid (DPA) with terbium ion (TbCl3) and fabricated a prototype of spore detection system. The detector measures the fluorescence emission of DP A․TbCl3 in the Bacillus spores. The detection system consists of a air sampling device, band-pass filters and a photomultiplier tube array, which measures fluorescence intensity in the wavelength band from 550 nm to 565 nm. The DPA․TbCl3 complex has fluorescence emission band arround 560 nm, when it is excited by UV light in the excitation wavelength range around 280 nm. The experimental parameters investigated spores concentrated form air, amount of DPA release from Bacillus subtilis spores by dodecylamine solution. The limit of detection was 1000 CFU/ml.
저자
Ok-Jae Sohn [ Department of Material and Biochemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Post-BK 21 Chonnam National University ]
Chang Whan Jung [ BioProcess Technologylab., Research Center for BioPhotonics, Post-BK21 ]
Jong Il Rhee [ School of Applied Chemical Engineering, BioProcess Technology lab., Research Center for BioPhotonics, Post-BK 21 Chonnam National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동