Earticle

초록

영어

L-homophenylalanine is a building block of Angiotensin-Converting Enzyme(ACE) inhibitor and 2-phenylethanol is a most widely used aroma compound with rose-like odor. L-homophenylalanine and 2-phenylethanol were synthesized simultaneously from 2-oxo-4-butanoic acid and L-phenylalanine by coupling reaction of aminotransferase and the Ehrlich pathway using a recombinant E.coli. By adding on carbonyl reductase cofactor regeneration system, high yield L-homophenylalanine and 2-phenylethanol synthesis reaction is available. To select carbonyl reductase, the activities of six carbonyl reductases from Saccharomyces cerevisiae were assayed. Among those candidates, YGL157w showed the highest activity on phenylacetaldehyde. Phenylpyruvate decarboxylase was recruited from the Ehrlich pathway of Saccharomyces cerevisiae, which is the metabolic pathway of L-phenylalanine. In addition, regeneration of an expensive cofactor NADPH is achieved using Glucose dehydrogenase from Bacillus subtilis. Despite of the low kcat value of decarboxylase, 39.2mM of 2-phenylethanol was produced via whole cell reaction although we have used 1mM of NADPH.

저자

• Joon-Young HWANG [ School of Chemical and Biological Engineering, Seoul National University, Seoul ]
• Jihyang PARK [ School of Chemical and Biological Engineering, Seoul National University, Seoul ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, Seoul National University, Seoul ]

참고문헌

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576