Jae-Il Ahn, In-Keun Jang, Young-Kwon Seo, Hee-Hoon Yoon, Yeon-Ho Shin, Yang-Hwan Ryu, Jae-Chan Kim, Kye-Yong Song, Eun-Kyung Yang, Ki-Ho Kim, Jung-Keug Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98547
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
The reconstruction of rabbit corneal epithelium was performed through differentiation culture method using self-manufactured amniotic membrane (AM) supporter and lyophilized amniotic membrane (LAM). To evaluate the reconstructed corneal epithelium, we stained the reconstructed tissue sections against CK3, PCNA, and examined TEM. Tilting dynamic culture was better than static culture in terms of reconstructed corneal cell layers and proliferating activity for reconstructing corneal epithelium on LAM. Blinking dynamic culture, comparing with static culture, showed similar properties with rabbit cornea in stratification and differentiation of corneal epithelial cells under histological examination. The corneal epithelium was successfully reconstructed by the use of LAM and self-manufactured AM supporter by blinking dynamic culture. The reconstructed corneal epithelium in this experimental study is considered to be a good in vitro model in autograft and allograft for treating the patients with severely damaged corneal surface and allograft for treating damaged skin and mucosa.
저자
Jae-Il Ahn [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
In-Keun Jang [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
Young-Kwon Seo [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
Hee-Hoon Yoon [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
Yeon-Ho Shin [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
Yang-Hwan Ryu [ Department of Ophthalmology, Chung-Ang University Hospital, Korea ]
Jae-Chan Kim [ Department of Ophthalmology, Chung-Ang University Hospital, Korea ]
Kye-Yong Song [ Department of Pathology, Chung-Ang University College of Medicine, Korea ]
Eun-Kyung Yang [ R&D Center, Bioland Ltd., Korea ]
Ki-Ho Kim [ R&D Center, Bioland Ltd., Korea ]
Jung-Keug Park [ Department of Chemical and Biochemical Engineering, Dongguk University, Korea ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동