Earticle

초록

영어

A cell-once-through (COT) perfusion concept was applied to a dual bioreactor system coupled to a Centritech Lab II Centrifuge for culture of recombinant Chinese hamster ovary (rCHO) cells for monoclonal antibody production. In this new culture mode, i.e., the COT perfusion process, total spent medium was transferred the centrifuge and a fixed percentage was removed. Accordingly, a significant reduction of the cell-passage frequency to the centrifuge led to minimization of cell damage caused by mechanical shear stress. Although the suppressive effects of low culture temperature on cell growth led to a loss of stability in a long-term COT perfusion culture system, the average antibody concentration at 33°C was 157.8 mg/L, approximately 2.4-fold higher than that at 37°C. By the use of a fortified medium at 37°C, rCHO cells were maintained at high density above 1.2 × 107 cells/mL, and antibody was produced continuously in a range of 260 to 280 mg/L in a stable long-term COT perfusion culture. The proposed new culture mode, the COT perfusion approach, guarantees the recovery of rCHO cells damaged by lowered temperature or high lactate and ammonium concentration.

저자

• Byoung Jin Kim [ epartment of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ]
• Duk Jae Oh [ Department of Bioscience and Biotechnology, Sejong University ]
• Ho Nam Chang [ Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576