Earticle

초록

영어

Enzymatic steps from two different biosynthetic pathways were combined in E. coli, directing the
synthesis of a new class of biomolecules – ubiquinones with desaturated prenyl side chains. This was achieved by the activity of a C30 carotenoid desaturase CrtN from S. aureus, which exhibited an inherent flexibility in substrate recognition when compared to other carotenoid desaturases. By utilizing the known plasticity of the E. coli ubiquinone biosynthesis pathway and the unusual activity of CrtN, modified ubiquinone structures with desaturated prenyl side chains were generated. The side chains of the new structures were confirmed to have different degrees of desaturation by mass spectrometry and NMR analysis. In vivo 14C- labeling and in vitro activity studies showed that CrtN desaturates octaprenyl dipohosphates but not the ubiquinone compounds directly. Antioxidant properties of desaturated side-chain ubiquinones were analyzed in an in vitro β-carotene-linoleate model system and found to be higher than the corresponding unmodified ubiquinones. These results demonstrate that by combining pathway steps from different branches of biosynthetic networks classes of compounds not observed in nature can be synthesized, and structural motifs that are functionally important can be combined or enhanced

저자

• 이평천 [ 아주대학교 분자과학기술학과 ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576