Earticle

초록

영어

Enzyme-linked immunosorbent assay (ELISA) using enzyme-conjugated antibody is the
most popular detection method for biochemical materials and can be utilized for a wide variety
of analytes. In recent years, rapid, automatic, and sensitive detection methods of ELISA have
been required because of the increasing number of biological samples in clinical, environmental,
and bioindustrial fields. For these purposes, several methods, including one-step ELISAs and
membrane blotting immunoassays, have been proposed. Among them, an open sandwich assay, a one-step ELISA using affinity tag and an immunoliposome blotting assay will be introduced.
The open sandwich method depends on the antigen-dependent re-association of antibody
variable domains, VH and VL [1]. Each domain is coupled with a moiety of an interacting pair,
and re-association of VH and VL domains completes the formation of the pair. For example,
mutants of N- and C-terminal deleted β-galactosidase were genetically conjugated to VH and VL
domains, and appearance of β-galactosidase activity was detected. In one-step ELISA using
affinity tag, an antibody was conjugated with an affinity peptide tag (RAFIASRRIKRP) having
a specific affinity for a hydrophilic polystyrene [2]. This tag-conjugated antibody and an
enzyme-conjugated antibody were used in sandwich immunoassay and resulted in a substantial
reduction in operational time with a higher sensitivity compared with the conventional ELISA.
Immunoliposomes encapsulating color markers were used for membrane blotting immunoassay
[3]. Multipoint interaction between antigen and antibody and higher signal intensity increased
the sensitivity of the membrane blotting immunoassay by two orders of magnitude even at the
blotted sample volume of 2 μl.

저자

• Shigeo Katoh [ Dept. of Chemical Engineering Hanyang University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576