The covalent coupling of antibodies to solid surfaces or other biological/chemical molecules is a key step for the development of immune-based assays such as biosensors and antibody arrays. The choice of coupling method significantly affects subsequent antibody-antigen interactions. In order to fully maintain antigen binding abilities of modified antibodies, modifications must be site-selective and away from antigen binding sites, providing well-oriented antibodies. Antibody binding protein, protein G specifically binds the Fc region of an antibody and therefore provides proper orientation of the bound antibody, resulting in the antigen binding sites optimally-exposed to the assay solution without the need of any antibody modifications. Here we introduce several novel linker systems which utilize engineered protein G constructs for antibody immobilization. Various genetic and/or chemical modifications of protein G allowed controlled and efficient immobilization of antibodies on the surfaces of different assay platforms, such as those of glass/gold chips and nanoparticles.
저자
Yongwon Jung [ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ]
Jeong Min Lee [ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ]
Bong Hyun Chung [ BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동