There have been many attempts to develop sensitive and accurate techniques for the detection and diagnosis of pathogenic bacteria using nucleic acid-based technology. To achieve efficient simultaneous detection of eleven selected pathogens, we constructed oligonucleotide microarray containing double specific capture probes and developed a statistical pattern mapping analytical tool for pathogenic bacteria detection. The two capture probes were designed from the total variable regions of 16S rDNA for precise detection. This microarray system harboring double capture probes and pattern recognition showed precise subtype discrimination between two closely related species. Therefore, using the proposed oligonucleotide microarray, we could successfully classify species and even subtypes of food-borne pathogens simultaneously.
저자
Hyung Joon Cha [ National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology ]
Beong Hee Hwang [ National Research Laboratory of Molecular Biotechnology, Department of Chemical Engineering, Pohang University of Science and Technology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
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