Gun-Hee Kwon, Woo-Ju Jeong, Ae Ran Lee, Jeong Hwan Kim, Jae-Yong Park, Jaeho Cha, Young-Sun Song
언어
영어(ENG)
URL
https://www.earticle.net/Article/A98229
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원문정보
초록
영어
The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using aBacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantlycompared to B. licheniformis 10716 control cell. During the 100hr incubation in Luria-Bertaini broth at 37oC, fibrinolyticactivity of B. licheniformis TF increased rapidly at the late growth stage, after 52hr of incubation, which was confirmed byzymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media,60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.
목차
Abstract Introduction Materials and Methods Results and Discussion Acknowledgments References
Gun-Hee Kwon [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Woo-Ju Jeong [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Ae Ran Lee [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Jae-Yong Park [ Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Jaeho Cha [ Department of Microbiology, Pusan National University, Busan 609-735, Korea ]
Young-Sun Song [ School of Food and Life Science, Inje University, Gimhae, Gyeongnam 621-749, Korea ]
Jeong Hwan Kim [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
Corresponding author