Earticle

초록

영어

The inhibitory effects of naringenin, kaempherol, and apigenin on the production of cholesterol in HepG2 KCLB88065 and MCF-7 KCLB 30022 cells were evaluated. In this study, quercetin was used as a reference reagent. Afterincubation for 3 days, fat-soluble contents of both cell types were extracted by using the Folch method and the cholesterolcontents in both cultured cells were determined by high performance liquid chromatography. The concentration of cholesterolin untreated each tissue cells was 12.2±0.11 and 8.83±0.12mg/g of lipid, respectively. The total concentration of eachflavonoid was adjusted to 0, 35, or 350µM in the culture broth. As the results, the addition of 2% methanol and dimethylsulfoxide (DMSO) to the media (control for flavonoid solvents) did not significantly affect cell growth; however, DMSOcaused an increase in the production of cholesterol. Each flavonoid inhibited the production of cholesterol in both HepG2 andMCF-7 cells at the concentration of 35µM above. In addition, the inhibitory effect of kaempherol on the production ofcholesterol in these cells was greater than the other flavonoids tested and HepG2 cells are more sensitive to flavonoids thanMCF-7. From the results, the inhibitory effects of flavonoids on cholesterol production are different depending on the celltype.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자

• Kee-Tae Kim [ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea ]
• Eun-Ju Yeo [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Sun-Hee Moon [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Ssang-Goo Cho [ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea ]
• Ye-Sun Han [ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea ]
• Seung-Yeol Nah [ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea ]
• Hyun-Dong Paik [ Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664