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Simultaneous Detection of Food-borne Pathogenic Bacteria in Ready-to-eat Kimbab Using Multiplex PCR Method

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 17 Number 6 (2008.12)바로가기
  • 페이지
    pp.1240-1245
  • 저자
    Kye Man Cho, Woo Jin Lim, Devaiah M Kambiranda, Renukaradhya K Math, Seong Weon Kim, Su Young Hong, Han Dae Yun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A98203

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원문정보

초록

영어
Kimbab is the most popular ready-to-eat (RTE) food in Korea. A rapid detection method based on multiplex PCRtechnique was developed for detection of major food-borne pathogens like Salmonella spp., Shigella spp., Bacillus cereus,Listeria monocytongenes, and Staphylococcus aureus. Specific bands were obtained as 108 bp (Sau, S. aureus), 284 bp (Sal, S.enterica, S. enteritids, and S. typhmurium), 404 bp (Lmo, L. monocytogenes), 475bp (Bce, B. cereus), and 600 bp (Shi, S.flexineri and S. sonnei). Visible cell numbers varied from 4.14-5.03, 3.61-4.47, and 4.10-5.11 log CFU/g in randomly collectedJune, July, and August samples, respectively. Among the 30 kimbab samples obtained 83.3% samples were contaminated and16.7% samples were free from contamination. The highest rate of contamination was with S. aureus (56.7%) followed by B.cereus (43.3%), Salmonella spp. (36.7%), Shigella spp. (13.3%), and L. monocytogenes (6.7%). The identification of thepathogenic species could be faster using one polymerase chain reaction (PCR) and the ability to test for food-borne pathogenicspecies in kimbab will save time and increase the ability to assure its quality.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

ready-to-eat food kimbab food-borne pathogenic bacteria multiplex polymerase chain reaction (PCR) food safety

저자

  • Kye Man Cho [ Research Institue of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Devaiah M Kambiranda [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Seong Weon Kim [ Department of Food Science, Jinju National University, Jinju, Gyeongnam 660-758, Korea ]
  • Renukaradhya K Math [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Woo Jin Lim [ Research Institue of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ]
  • Su Young Hong [ Hazardous Substances Division, National Institute of Agricultural Science and Technology, Suwon, Gyeonggi 441-707, Korea ]
  • Han Dae Yun [ Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 6

    피인용수 : 0(자료제공 : 네이버학술정보)

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