Earticle

초록

영어

Secondary metabolites from the fruit body of Phellinus linteus were evaluated for their proliferative effect onhuman osteoblast-like cells. 3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyl-tetraxolium bromide (MTT) assay and alkaline phosphatase(ALP) activity assay were used to assess the effect those isolates on the human osteoblast-like cell line (Saos-2). Activity-guided fractionation led to the isolation of ALP-activating phenolic compounds through the extraction of P. linteus, solventpartitioning, and repeated silica gel and octadecyl silica gel (ODS) column chromatographic separations. From the result ofspectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry (MS), and infrared spectroscopy (IR), thechemical structures of the compounds were determined as 4-(4-hydroxyphenyl)-3-buten-2-one (1), 2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), 3,4-dihydroxybenzaldehyde (4), and protocatechuicacid methyl ester (5), respectively. This study reports the first isolation of compounds 1-3 and 5 from P. linteus. In addition, allphenolic compounds stimulated proliferation of the osteoblast-like cells and increased their ALP activity in a dose-dependentmanner (10-8 to 10-1 mg/mL). The present data demonstrate that phenolic compounds in P. linteus stimulated mineralization inbone formation caused by osteoporosis. The bone-formation effect of P. linteus seems to be mediated, at least partly, by thestimulating effect of the phenolic compounds on the growth of osteoblasts.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgment
 References

키워드

저자

• Ha-Na Lyu [ Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Gyeonggi 446-701, Korea ]
• Dae-Young Lee [ Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Gyeonggi 446-701, Korea ]
• Dong-Hyun Kim [ School of Chemistry, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M17DN, UK ]
• Jong-Su Yoo [ Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Gyeonggi 446-701, Korea ]
• Min-Kyung Lee [ Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Gyeonggi 446-701, Korea ]
• Lee, In-Ho Kim [ Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea ]
• Nam-In Baek [ Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Yongin, Gyeonggi 446-701, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664