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Effect of Osmolarity of Culture Medium on Imprinting and Apoptotic Gene Expression in Miniature Pig Nuclear Transfer Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 32 No 3 (2008.09)바로가기
  • 페이지
    pp.183-191
  • 저자
    Mi-Rung Park, In-Sun Hwang, Joo-Hyun Shim, Hyo-Jin Moon, Dong-Hoon Kim, Yeoung-Kyu Ko, Hwan-Hoo Seong, Gi-Sun Im
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A88589

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원문정보

초록

영어
This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax- and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Oocyte Recovery and Maturation
  Preparation of Nuclear Donor Cells
  Production of Nuclear Transfer Embryos
  Terminal DeoxynucleotidyI Transferase-Mediated dUTP Nick-End Labeling (TUNEL) Assay
  Collection of In Vivo Blastocysts
  Realtime PCR Quantification
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Miniature pig NT embryos Osmolarity Gene expression

저자

  • Mi-Rung Park [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • In-Sun Hwang [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Joo-Hyun Shim [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Hyo-Jin Moon [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Dong-Hoon Kim [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Yeoung-Kyu Ko [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Hwan-Hoo Seong [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Gi-Sun Im [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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