Earticle

초록

영어

This study was conducted to investigate an effective recipient oocyte and culture system for producing of Hanwoo (Korean native cattle) somatic cell nuclear transfer (SCNT) embryos. Hanwoo ear skin fibroblasts were used as donor cells. In vitro matured Hanwoo or Holstein oocytes were enucleated, and single donor cells were transferred into the perivitelline space of the enucleated oocytes. The couplets were subsequently fused and activated. The reconstructed embryos were cultured in a conventional or sequential culture system. In the former, embryos were cultured in CR2aa medium for eight days; in the latter, embryos were cultured in modified CR2aa-A (mCR2-A) for three days and then further cultured in modified CR2aa-B (mCR2-B) for five days. In the experiment with the recipient oocyte, the rate of embryo development to the blastocyst stage was significantly (p<0.05) higher in Hanwoo recipient oocytes than in Holstein ones (48.8% vs 38.9%). BIastocysts derived from Hanwoo recipient oocytes contained significantly (p<0.05) higher numbers of total cells than those derived from Holstein recipient oocytes (156.0+-68.2 vs 134.7+-54.8)). There was no difference in the mean proportion of apoptotic cells in blastocysts between the sources of recipient oocytes. In the experiment with the embryo culture system, the blastocyst rate was somewhat higher in sequential system than in conventional system (50.0% vs 43.5%), though there was no significant difference. The numbers of total (160.0+-69.0 vs 156.7+-68.4) and apoptotic cells (14.0+-10.4 vs 11.8+-6.4)) were not different between the culture systems. In conclusion, the present study demonstrated that Hanwoo recipient oocytes and the sequential culture system were more effective in supporting the production of Hanwoo SCNT embryos.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Bovine Oocytes
  Preparation of Somatic Cells
  Enucleation and Nuclear Transfer
  Fusion and Activation
  In Vitro Culture of Embryos
  Terminal Deoxynucleotidyl  Transferase-Mediated dUTP Nick-End Labeling (TUNEL) Assay
  Statistical Analysis
 RESULTS
  Development of Hanwoo SCNT Embryos according to Recipient Oocyte Source
  Development of Hanwoo SCNT Embryos according to Culture Systems
 DISCUSSION
 REFERENCES

키워드

저자

• Dong-Hoon Kim [ Animal Biotechnology Division, Natioanl Institute of Animal Science ] Corresponding author
• Se-woong Kim [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Min-Jung Lee [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Seong-Hoon Bae [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Gi-Sun Im [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Hyun-Joo Lim [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Byoung-Chul Yang [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]
• Hwan-Hoo Seong [ Animal Biotechnology Division, Natioanl Institute of Animal Science ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636