Earticle

초록

영어

Somatic cells such as oviduct epithelial cell, uterine epithelial cell, cumulus-granulosa cell and buffalo rat river cell has been used to establish an effective culture system for bovine embryos produced in in vitro. But nitric oxide (NO) metabolites secreted from somatic cells were largely arrested the development of bovine in vitro matured/ in vitro fertilized (IVM/IVF) embryos, suggesting that NO was induced the embryonic toxic substance into culture medium. The objective of this study was to investigate whether BOEC co-culture system can ameliorate the NO-mediated oxidative stress in the culture of bovine IVM/IVF embryos. Therefore, we evaluated the developmental rate of bovine IVM/IVF embryos under BOEC co-culture system in the presence or absence of sodium nitroprusside (SNP), as a NO donor, and also detected the expression of growth factor (TGF-p , EGF and IGFBP) and apoptosis (Caspase-3, Bax and Bcl-2) genes. The supplement of SNP over 5 uM was strongly inhibited blastocyst development of bovine IVM/IVF embryos than in control and 1 uM SNP group (Table 2). The developmental rates beyond morulae stages of bovine IVM/IVF embryos co-cultured with BOEC regardless of SNP supplement (40.4% in 5 uM SNP+ BOEC group and 65.1% in BOEC group) were significantly increased than those of control (35.0%) and SNP single treatment group (23.3%, p<0.05: Table 3). The transcripts of Bax and Caspase-3 genes were detected in all experiment groups (1:Isolated fresh cell (IFC), 2:Primary culture cell (PCC), 3:PCC after using the embryo culture, 4: PCC containing 5 uM SNP and 5: PCC containing 5 uM SNP after using the embryo culture), but Bcl-2 gene was not detected in IFC and PCC (Fig. 1). In the expression of growth factor genes, TGF-p gene was found in all experimental groups, and EGF and IGFBP genes were not found in IFC and PCC (Fig. 2). These results indicate that BOEC co-culture system can increase the development beyond morula stages of bovine IVM/IVF embryos, possibly suggesting the alleviation of embryonic toxic substance like nitric oxide.

목차

ABSTRACT
 INTRODUCTION
 MATERIAL AND METHODS
  In Vitro Production of Bovine IVM/IVF Embryos
  Isolation and Primary Culture of Bovine Oviduct Epithelial Cell (BOEC)
  RNA Isolation of BOEC
  Reverse-transcription Poly merase Chain Reaction (RTPCR) Analysis
  Statistical Analysis
 RESULTS
 DISCUSSION
 ACKNOWLEDGEMENT
 REFERENCES

키워드

저자

• Hyun-Yong Jang [ College of Animal Life Science ]
• Yu-Sung Jung [ College of Animal Life Science ]
• Zheng-Yi Li [ College of PhAmAcy, Kangwon National University ]
• Hyoung-Jong Yoon [ College of PhAmAcy, Kangwon National University ]
• Hee-Tae Cheong [ School of Veterinary Medicine and College of Phamacy, Kangwon National University ]
• Jong-Taek Kim [ School of Veterinary Medicine and College of Phamacy, Kangwon National University ]
• Choon-Keun Park [ College of Animal Life Science ]
• Boo-Keun Yang [ College of Animal Life Science ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636