The structural change and leakage of cellular substances of Saccharomyces cerevisiae attributed by high hydrostatic pressure (HHP) treatment were observed with scanning electron microscopy (SEM). S. cerevisiae (ATCC16664) was inoculated in apple juice for 10 min at 23°C and the apple juice treated at 138, 207, 276, 345, and 414 MPa pressure for 30 sec at 23oC. Increased of roughness, elongation, wrinkling, and pores on yeast cell surfaces, the yeast cell walls were severely damaged by HHP treatment from 276 to 414 MPa. Inactivation of S. cerevisiae by HHP is dependent on structural changes on the cell walls observed with SEM.
목차
Abstract Introduction Materials and Methods S. cerevisiae preparation High hydrostatic pressure (HHP) treatment Viability of S. cerevisiae Scanning electron microscopy (SEM) Results and Discussion Viability of S. cerevisiae before and after HHP treatment SEM of S. cerevisiae before and after HHP treatment References
키워드
high hydrostatic pressure (HHP)Saccharomyces cerevisiaescanning electron microscopy (SEM)
저자
Woo-Suk Bang [ Department of Food Science, North Carolina State University ]
Corresponding author
Barry G. Swanson [ Department of Food Science and Human Nutrition, Washington State University ]