Earticle

초록

영어

The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. ‘Hanakkori’ materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In ‘Hanakkori’, 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-
methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and
glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).

목차

Abstract
 Introduction
 Materials and Methods
  Chemicals
  Plant materials
  Glucosinolate extraction and analysis
  Mass spectrometry (MS)
  Statistical analysis
 Results and Discussion
  Identification of GSLs
  Quantitative determination of GSLs
  Evaluation of the potentially bitter-tasting GSLs
  A GSL pattern of alkenyl GSLs
 References

키워드

저자

• Sun-Ju Kim [ Crop Functionality and Utilization Research Subteam, Upland Farming Research Station, National Agriculture Research Center for Hokkaido Region ] Corresponding author
• Kouei Fujii [ Department of Crop Breeding, Yamaguchi Prefecture Agricultural Research Institute ]
• Zaidul Islam Sarker Mohamed [ Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia ]
• Hyun-Woong Kim [ Life and Environmental Sciences Design, Faculty of Engineering, Chiba Institute of Technology ]
• Hiroaki Yamauchi [ Crop Functionality and Utilization Research Subteam, Upland Farming Research Station, National Agriculture Research Center for Hokkaido Region ]
• Gensho Ishii [ Local Crop Breeding Research Team, National Agricultural Research Center for Hokkaido Region ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664