Earticle

초록

영어

Commercial food products from major cities of Coahuila, Mexico were screened to identify residues of transgenic deoxyribonucleic acid (DNA) and/or proteins. After performed, an inventory on all products that contained a soybean-based ingredient in a commercial grocery store in the city of Saltillo, Coahuila, Mexico, 245 food products were identified and grouped in 15 classes according to the soybean ingredient as well as the manufacturing process used for their elaboration.
Similar sampling was made for the different food classes in the cities of Monclova, Piedras Negras, and Torreon. A total of 88 samples were analyzed and DNA was extracted by the hexadecyltrimethyl-ammonium bromide (CTAB) technique with slight modification to obtain better DNA quality (1). In addition, segments of the transgenic genes one that codifies for 5-
enolpyruvylshikimate-3-phosphate synthase (epsps), cry 1A, and the cauliflower mosaic virus (CaMV) promoter were amplified using polymerase chain reaction (PCR). The transgenic proteins 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) and insecticidal crystal protein (Cry 1Ab/Ac) were identified using double antibody sandwich-enzymatic linked
immunoassay analysis (DAS-ELISA). Presence of transgenic genes and/or proteins was identified in 35.3% of the commercial products samples.

목차

Abstract
 Introduction
 Materials and Methods
  Food samples
  DNA isolation
  PCR reaction and product analysis
  Presence of transgenic proteins in commercial soybeanfoods from Mexican grocery stores
 Results and Discussion
  Modifications done to the DNA extraction method
  Presence of 35s CaMV promoter, epsps and cry 1ABgenes in commercial soybean foods
  Presence of transgenic proteins in commercial soybeanfoods from Mexican grocery stores
 References

키워드

저자

• Yendi Arely Cruz-Flores [ School of Chemistry, Universidad Autónoma de Coahuila, V. Carranza e Ing. ]
• Raúl Rodríguez-Herrera [ School of Chemistry, Universidad Autonoma de Coahuila, V. Carranza e Ing. ] Corresponding author
• Cristóbal Noe Aguilar-González [ School of Chemistry, Universidad Autonoma de Coahuila, V. Carranza e Ing. ]
• Juan Carlos Contreras-Esquivel [ School of Chemistry, Universidad Autonoma de Coahuila, V. Carranza e Ing. ]
• Maria de la Luz Reyes-Vega [ School of Chemistry, Universidad Autonoma de Coahuila, V. Carranza e Ing. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664