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In vitro Antioxidant and Cytoprotective Activities of the Extract of Dangyuja (Citrus grandis Osbeck) Leaves

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 17 Number 5 (2008.10)바로가기
  • 페이지
    pp.1086-1091
  • 저자
    Yunjung Kim, Moonjae Cho, Somi Kim Cho
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A88437

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원문정보

초록

영어
The antioxidant activities of the extracts of dangyuja (Citrus grandis Osbeck) leaves were evaluated. The highest phenolic content was obtained from the ethyl acetate fraction (EF) (202.1±0.8 mg GAE/g dried extract) and it exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The cytoprotective effects of EF on oxidative
damage induced by tert-butyl hydroperoxide (t-BHP) in a human hepatoma cell line, HepG2 cells, were investigated to understand the intracellular antioxidant mechanisms. Treatment of HepG2 cells with EF prior to oxidative stress was found to inhibit reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage in a dose-dependent manner. Gas
chromatography-mass spectrometry (GC-MS) studies on EF resulted in tentative identification of 19 compounds representing 94.3% of the total content. Taken together, these results demonstrated that EF has excellent antioxidant activities and thus dangyuja leaves have great potential as a source for natural antioxidant which can be applied in food products.

목차

Abstract
 Introduction
 Materials and Methods
  Materials
  Preparation of extracts
  Determination of total phenolics and flavonoids
  Gas chromatography
  GC-MS
  Free radical scavenging activity
  Cell culture
  Determination of ROS generation
  Measurement of lipid peroxidation
  Determination of DNA damage (Comet assay)
  Cytotoxicity assay
  Statistical analysis
 Results and Discussion
  Total phenolic content and total flavonoid content
  DPPH free radical scavenging activity
  Effect of EF on t-BHP-induced intracellular ROSgeneration
  Compositional analysis of EF
 References

키워드

Citrus grandis Osbeck antioxidant DPPH radical scavenging tert-butyl hydroperoxide HepG2 cell

저자

  • Yunjung Kim [ Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University ]
  • Moonjae Cho [ Department of Medicine, Medical School, Cheju National University ]
  • Somi Kim Cho [ 1Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University, The Research Institute for Subtropical Agriculture and Biotechnology, Cheju National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 5

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