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Up-regulation of Aldo-keto Reductase 1C3 Expression in Sulforaphane-treated MCF-7 Breast Cancer Cells
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- 발행기관
- 한국식품과학회 바로가기
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- 간행물
- Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
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- 통권
- Volume 17 Number 5 (2008.10)바로가기
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- 페이지
- pp.1079-1085
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A88436
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- The chemopreventive activity of sulforaphane (SFN) occurs through its inhibition of carcinogen-activating enzymes and its induction of detoxification enzymes. However, the exact mechanisms by which SFN exerts its anti-carcinogenic effects are not fully understood. Therefore, the mechanisms underlying the cytoprotective effects of SFN were examined in MCF-7
breast cancer cells. Exposure of cells to SFN (10 μM) induced a transcriptional change in the AKR1C3 gene, which is one of aldo-keto reductases (AKRs) family that is associated with detoxification and antioxidant response. Further analysis revealed that SFN elicited a dose- and time-dependent increase in the expression of both the NRF2 and AKR1C3 proteins. Moreover,
this up-regulation of AKR1C3 was inhibited by pretreatment with antioxidant, N-acetyl-L-cysteine (NAC), which suggests that the up-regulation of AKR1C3 expression induced by SFN involves reactive oxygen species (ROS) signaling. Furthermore, pretreatment of cells with LY294002, a pharmacologic inhibitor of phosphatidylinositol 3-kinase (PI3K), suppressed the SFNaugmented
Nrf2 activation and AKR1C3 expression; however, inhibition of PKC or MEK1/2 signaling with Gö6976 or PD98059, respectively, did not alter SFN-induced AKR1C3 expression. Collectively, these data suggest that SFN can modulate the expression of the AKR1C3 in MCF-7 cells by activation of PI3K via the generation of ROS.
목차
Abstract
Introduction
Materials and Methods
Reagents and cell culture
MTT assay
Differential display
RT-PCR
Western blotting for AKR1C3 expression
Statistical analysis
Results and Discussion
SFN-treated MCF cells differentially express AKR1C3
SFN up-regulates the expression of AKR1C3 in a ROSandPI3K/AKT-dependent manner
References
키워드
sulforaphane AKR1C3 cytoprotection phosphatidylinositol 3-kinase reactive oxygen species저자
간행물 정보
발행기관
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- 발행기관명
- 한국식품과학회 [Korean Society of Food Science and Technology]
- 설립연도
- 1970
- 분야
- 농수해양>식품과학
- 소개
- 본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.
간행물
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- 간행물명
- Food Science and Biotechnology
- 간기
- 격월간
- pISSN
- 1226-7708
- 수록기간
- 1992~2009
- 십진분류
- KDC 574 DDC 664
이 권호 내 다른 논문 / Food Science and Biotechnology Volume 17 Number 5
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