Earticle

초록

영어

This study was performed to determine the effects of γ-irradiation on the antiallergic activity of alginate. An alginate aqueous solution was γ-irradiated at 3, 5, 7, 10, 20, and 100 kGy. First, the molecular weight (Mw) of alginate rapidly decreased as the γ-irradiation dose increased up to 20 kGy. Then, the antiallergic activity of the γ-irradiated alginate was measured. Interlukin (IL)-4 cytokine and IgE level were significantly decreased in the γ-irradiated alginate groups as
compared to the control group in vitro. The mice intraperitoneally administered with the γ-irradiated alginate exhibited lower ovalbumin-specific IgE and IgG1 level in serum than the control mice. Furthermore, the γ-irradiated alginate suppressed total and ovalbumin-specific IgE secretions in the splenocytes. Increased IL-2 level was observed in the culture supernatants of the splenocytes that were obtained from the mice administerd with the γ-irradiated alginate, while IL-4 level decreased. The present study indicates that γ-irradiated alginate can suppress allergy in a mouse allergy model.

목차

Abstract
 Introduction
 Materials and Methods
  Mice
  Reagent
  γ-Irradiation
  Gel permeation chromatography (GPC)
  Immunization protocol
  Spleen cell culture for IgE and cytokine production
  Determination of cytokine level
  Determination of ovalbumin-specific antibody level
  Splenocytes proliferation assay
  Statistical analysis
 Results and Discussion
  Effect of the γ-irradiation on the Mw of alginate
  Effect of the γ-irradiated alginate on IL-4 and IgE levelin vitro
  Effect of the γ-irradiated alginate on antibody level inthe serum
  Effect of the γ-irradiated alginate on cytokine level inthe splenocytes
  Effect of the γ-irradiated alginate on total andovalbumin-specific IgE in the splenocytes
  Effect of the γ-irradiated alginate on the splenocytesproliferation
 References

키워드

저자

• Eu Jin Song [ Faculty of Food Science and Biotechnology/Institute of Food Science, Pukyong National University ]
• So Young Lee [ Faculty of Food Science and Biotechnology/Institute of Food Science, Pukyong National University ]
• Koth-Bong-Woo-Ri Kim [ Faculty of Food Science and Biotechnology/Institute of Food Science, Pukyong National University ]
• Jin Gyu Park [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute ]
• Jae Hun Kim [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute ]
• Ju Woon Lee [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute ]
• Myung Woo Byun [ Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute ]
• Dong Hyun Ahn [ Faculty of Food Science and Biotechnology/Institute of Food Science, Pukyong National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664