Earticle

초록

영어

A thermostable α-L-arabinofuranosidases (α-L-AFase) is an industrially important enzyme for recovery of Larabinose from hemicellulose. The recombinant α-L-AFase from Thermotoga maritima was expressed in Escherichia coli by using a constitutive pHCE or an inducible pRSET vectors. In batch fermentation, the constitutive expression system resulted in slightly faster growth rate (0.78 vs. 0.74/hr) but lower enzyme activity (2,553 vs. 3,723 units/L) than those of the induction system. When fed-batch fermentation was performed, biomass and enzyme activity reached the highest levels of 36 g/L and 9,152 units/L, respectively. The fed batch cultures performed superior results than batch culture in terms of biomass yield (4.62-5.42 folds) and enzyme synthesis (3.39-4.00 folds). In addition, the fed-batch induction strategy at high cell density resulted in the best productivity in cell growth as well as enzyme activity rather than the induction method at low cell density or the constitutive expression.

목차

Abstract
 Introduction
 Materials and Methods
  Bacterial strains and plasmids
  Culture and media
  Batch and fed-batch fermentation
  Analytical methods
 Results and Discussion
  Batch culture and protein analysis
  Fed-batch culture with constitutive expression
  Fed-batch culture by induction at low cell density
  Fed-batch culture by induction at high cell density
 References

키워드

저자

• Jae Yong Song [ Department of Chemical Engineering, Chungbuk National University ]
• In-Kyung Keum [ Department of Food Science and Technology, Chungbuk National University ]
• Qing Jin [ Department of Food Science and Technology, Chungbuk National University, Department of Food Science, Yanbian University ]
• Jung-Mi Park [ Department of Food Science and Technology, Chungbuk National University ]
• Beom Soo Kim [ Department of Chemical Engineering, Chungbuk National University ]
• Bong Hwan Jung [ Department of Food Science and Technology, Chungbuk National University ]
• Tae-Jip Kim [ Department of Food Science and Technology, Chungbuk National University ]
• Nam Soo Han [ Department of Food Science and Technology, Chungbuk National University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664