Earticle

초록

영어

Two flavonoids, isorhamnetin 3-O-β-D-glucopyranoside (1) and quercetin 3-O-β-D-glucopyranoside (2), from slander glasswort (Salicornia herbacea, Korean name hamcho) were isolated. Antioxidative and matrix metalloproteinase-9 (MMP-9) inhibitory effects of these compounds were investigated in HT 1080 cell lines. These compounds suppressed the
electron spin resonance (ESR) signal intensity on generation of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical in a freecellular system. Their scavenging effects on generation of intercellular reactive oxygen species (ROS) also exhibited similar trends with DPPH radical in the free cellular system. Also, a control group combined only with Fe(II)-H2O2 resulted in DNA
apoptosis by oxidative stress, whereas treatments with these compounds suppressed radical-mediated DNA damage. Intracellular glutathione (GSH) levels were slightly increased in the presence of compound 1 and 2. Moreover, these compounds led to the reduction of the expression levels of MMP-9 without cytotoxic influence. These results suggest that these compounds have a potential as a valuable natural antioxidant and MMP inhibitor related to oxidative stress. Therefore, these compounds not only can be developed as a candidate for a therapeutic potential but also a source for use as ingredients of health foods or functional
foods to prevent metastasis involving MMP-9, closely related to ROS.

목차

Abstract
 Introduction
 Materials and Methods
  Plant material and isolation of compounds
  Determination of DPPH radical scavenging activity byelectron spin resonance spectroscopy
  Cell culture and cytotoxicity determination using MTTassay
  Determination of intracellular formation of ROS usingDCF-DA labeling
  Genomic DNA isolation
  Determination of radical-mediated DNA damage
  Measurement of intracellular GSH level
  Determination of MMP activity by gelatin zymography
  Statistical analysis
 Results and Discussion
  Scavenging activity on DPPH radicals
  Cell viability
  Cellular radical scavenging effect
  Inhibitory effect on DNA oxidation
  Regulation of GSH Level
  Inhibition effect on MMP-9 activity
 References

키워드

저자

• Chang-Suk Kong [ Research Institute of Marine Science and Technology, Korea Maritime University ]
• You Ah Kim [ Division of Marine Environment and Bioscience, Korea Maritime University ]
• Moon-Moo Kim [ 2Department of Chemistry, Dong-Eui University ]
• Jin-Sook Park [ Department of Chemistry, Pukyong National University ]
• Se-Kwon Kim [ Department of Chemistry, Pukyong National University ]
• Burm-Jong Lee [ Department of Chemistry, Inje University ]
• Taek Jeong Nam [ Department of Chemistry, Inje University ]
• Youngwan Seo [ Division of Marine Environment and Bioscience, Korea Maritime University ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664